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454 bu  (ATCC)


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    ATCC 454 bu
    454 Bu, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/454+bu/pmc09224431-197-4-6?v=ATCC
    Average 95 stars, based on 185 article reviews
    454 bu - by Bioz Stars, 2026-07
    95/100 stars

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    454 bu  (ATCC)
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    mcherry expressing c. trachomatis serovar l2 454/bu  (Thermo Fisher)
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    Thermo Fisher mcherry expressing c. trachomatis serovar l2 454/bu
    C. <t>trachomatis</t> expressing the red fluorescent protein <t>mCherry</t> can be detected without immunostaining. Images in (A) represents live infection imaging (upper) of Hoechst (blue) and mCherry (red). Lower panel images are acquired in the same field after immunostaining with FITC-labeled antibody. A merge of live and stained images is shown. Bars in (B) show the inclusion count obtained in in three individual wells from mCherry live imaging and FITC immunostained inclusions, respectively. (C) wt and mCherry expressing C. trachomatis are equally sensitive to doxycycline. Live imaging was used for mCherry expressing C. trachomatis inclusions while wt inclusions were visualized by FITC immunostaining.
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    Image Search Results


    C. trachomatis expressing the red fluorescent protein mCherry can be detected without immunostaining. Images in (A) represents live infection imaging (upper) of Hoechst (blue) and mCherry (red). Lower panel images are acquired in the same field after immunostaining with FITC-labeled antibody. A merge of live and stained images is shown. Bars in (B) show the inclusion count obtained in in three individual wells from mCherry live imaging and FITC immunostained inclusions, respectively. (C) wt and mCherry expressing C. trachomatis are equally sensitive to doxycycline. Live imaging was used for mCherry expressing C. trachomatis inclusions while wt inclusions were visualized by FITC immunostaining.

    Journal: Frontiers in Microbiology

    Article Title: Red Fluorescent Chlamydia trachomatis Applied to Live Cell Imaging and Screening for Antibacterial Agents

    doi: 10.3389/fmicb.2018.03151

    Figure Lengend Snippet: C. trachomatis expressing the red fluorescent protein mCherry can be detected without immunostaining. Images in (A) represents live infection imaging (upper) of Hoechst (blue) and mCherry (red). Lower panel images are acquired in the same field after immunostaining with FITC-labeled antibody. A merge of live and stained images is shown. Bars in (B) show the inclusion count obtained in in three individual wells from mCherry live imaging and FITC immunostained inclusions, respectively. (C) wt and mCherry expressing C. trachomatis are equally sensitive to doxycycline. Live imaging was used for mCherry expressing C. trachomatis inclusions while wt inclusions were visualized by FITC immunostaining.

    Article Snippet: The primary screen of the 349 open access compound library was performed using the mCherry expressing C. trachomatis serovar L2 454/Bu in clear, flat 96-well plates (Nunc, Delta surface, Thermo Fischer Scientific).

    Techniques: Expressing, Immunostaining, Infection, Imaging, Labeling, Staining

    EC 50 values with 95% confidence values for selected compounds against wt and mCherry expressing C. trachomatis .

    Journal: Frontiers in Microbiology

    Article Title: Red Fluorescent Chlamydia trachomatis Applied to Live Cell Imaging and Screening for Antibacterial Agents

    doi: 10.3389/fmicb.2018.03151

    Figure Lengend Snippet: EC 50 values with 95% confidence values for selected compounds against wt and mCherry expressing C. trachomatis .

    Article Snippet: The primary screen of the 349 open access compound library was performed using the mCherry expressing C. trachomatis serovar L2 454/Bu in clear, flat 96-well plates (Nunc, Delta surface, Thermo Fischer Scientific).

    Techniques: Expressing

    Dose-response curves of selected compounds against wt (A) and mCherry expressing (B) C. trachomatis . Both strains were visualized using FITC immunostaining. cmp, compound.

    Journal: Frontiers in Microbiology

    Article Title: Red Fluorescent Chlamydia trachomatis Applied to Live Cell Imaging and Screening for Antibacterial Agents

    doi: 10.3389/fmicb.2018.03151

    Figure Lengend Snippet: Dose-response curves of selected compounds against wt (A) and mCherry expressing (B) C. trachomatis . Both strains were visualized using FITC immunostaining. cmp, compound.

    Article Snippet: The primary screen of the 349 open access compound library was performed using the mCherry expressing C. trachomatis serovar L2 454/Bu in clear, flat 96-well plates (Nunc, Delta surface, Thermo Fischer Scientific).

    Techniques: Expressing, Immunostaining